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Nondenaturing conditions were required to investigate the complex of pheromone and pheromone binding protein (PBP) from the silkworm moth Bombyx mori by electrospray ionization mass spectrometry (ESI-MS). The technique revealed information about the binding specificity of the sex attractant bombykol 1 and other ligands.
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Oldham et al. (2000) studied this question.
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