Key result
The hSlo alpha-subunit efficiently reaches the apical cell surface independent of N-glycosylation.
Population
Permanently transfected Madin-Darby canine kidney cells expressing human K alpha-subunit
Design
Preclinical
Authors
Loading...
May guide epithelial ion channel trafficking studies; leaves open raft-mediated sorting in native mammalian models.
The apical sorting of the human Slowpoke channel in MDCK cells is independent of N-glycosylation and may involve association with lipid rafts.
Bravo‐Zehnder et al. (2000) studied this question. hSlo channel mutation (lacking N-glycosylation site) and tunicamycin treatment vs. Wild-type hSlo and untreated cells was evaluated on Apical sorting and segregation of hSlo. The human Slowpoke channel (hSlo) alpha-subunit is efficiently transported to the apical cell surface in Madin-Darby canine kidney cells independent of N-glycosylation.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: