Development of a bioassay for putative human lymphoma stem cells

studied prior to treatment, 22 at the time of relapse, and the others while in remission.Of the 65 patients, 27 had nodular lymphoma (lymphocytic, 23; mixed cell type, 2; histiocytic, 2); 38 had diffuse lymphoma (lymphocytic, poorly differentiated, 22; lymphocytic, well differentiated, 5; histiocytic, 7; lymphoblastic [T cell), 2; undifferentiated, 1; "hairy cell leukemia," 1).The majority of cultures for lymphoma stem cells were performed on bone marrow aspirates obtained simultaneously with bone marrow aspirates and core biopsies that were routinely acquired for initial staging,7 restaging to assess