Hybridization of Half Molecules of Rabbit Gamma Globulin

Specifically purified rabbit antiovalbumin and normal gamma-globulin labeled with 1-131 were dissociated into half molecules by reduction and acidification. When a mixture of the two preparations was neutralized, a large proportion of mixed molecules having active combining sites and the same sedimentation coefficient as the original gamma-globulins was formed. Since the sulfhydryl groups were inactivated after reduction, the recombined subunits appear to be linked by noncovalent bonds.