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August 21, 2002Acta Anaesthesiologica Scandinavica

Prostaglandin E1 at clinically relevant concentrations inhibits aggregation of platelets under synergic interaction with endothelial cells

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Key result

Prostaglandin E1 lowers the bradykinin concentration required to inhibit human platelet aggregation by ~89%.

Why the study?

Does prostaglandin E1 at clinically relevant concentrations inhibit platelet aggregation through synergism with endothelial cells?

Population

Human platelets and cultured porcine aortic endothelial (PAE) cells

Comparison

Prostaglandin E1 at clinically relevant… vs PGE1 alone or PAE cell incubation buffer alone

Design

Preclinical

Authors

TKTakuro KogaTAToshiharu AzmaOYOsafumi Yuge

Discussion

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Overview

May potentiate bradykinin-mediated platelet inhibition; leaves open endothelial synergy and clinical relevance in humans.

Structured PICO

Does prostaglandin E1 at clinically relevant concentrations inhibit platelet aggregation through synergism with endothelial cells?

P
Population
Human platelets and cultured porcine aortic endothelial (PAE) cells
I
Intervention
Prostaglandin E1 (PGE1) at clinically relevant concentrations (0.1 to 1 ng/ml) combined with incubation buffer from PAE cells stimulated by bradykinin
C
Comparator
PGE1 alone or PAE cell incubation buffer alone
O
Outcome
Inhibition of human platelet aggregation induced by 2 micro g/ml collagen measured by turbidimetrysurrogate

Main Result

Absolute Event Rate: 10.3% vs 95.4%

PGE1 at clinically relevant concentrations (>=0.1 ng/ml) inhibits platelet aggregation through a synergistic interaction with endothelial cell-derived factors, particularly nitric oxide.

Cite This Study

Koga et al. (2002) studied Platelet aggregation. Prostaglandin E1 (PGE1) vs. Bradykinin alone was evaluated on Half-maximum effective concentration of bradykinin to inhibit aggregation. Prostaglandin E1 at clinically relevant concentrations (≥0.1 ng/ml) significantly decreased the half-maximum effective concentration of bradykinin required to inhibit human platelet aggregation.

synapsesocial.com/papers/6aa4bdcfac77d5296f2bc98ahttps://doi.org/10.1034/j.1399-6576.2002.460810.x
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