Fluorescence Recovery After Photobleaching (FRAP) is used to monitor the relaxation of concentration profiles after various imposed concentration fields. A theoretical analysis is conducted to quantify convective and diffusive transport and determine their contributions to fluorescence recovery in a perfused medium. Four different photobleach geometries—line, periodic pattern, uniform circular spot and Gaussian spot—are studied. It is shown that a spatially-averaged fluorescence profile (as would be the output of a photomultiplier) is inadequate in distinguishing convection from diffusion except in cases of large convection. In order to characterize both diffusive and convective effects, it is necessary to study the detailed concentration profile as a function of space and time.
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Srikanth Ranga Chary (1987) studied this question.
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