Sporulation in Protoplasts and Its Dependence on Prior Forespore Development

Fitz-James, Philip C. (University of Western Ontario, London, Ontario, Canada). Sporulation in protoplasts and its dependence on prior forespore development. J. Bacteriol. 87:667-675. 1964.-Phase-contrast and electron microscopy were used to decide whether cells from late growth or sporulating cultures could, where converted into protoplasts, complete the process of sporulation. The cell wall was found essential to the development of the forespore. Incomplete forespores formed a satellite protoplast. Completed forespores were enclosed by the sporangial protoplast, and only these or later forms were able to complete sporulation in protoplasts.