Impaired graft survival in pediatric renal transplant recipients with donor-specific antibodies detected by solid-phase assays

Verghese PS, Smith JM, McDonald RA, Schwartz SM, Nelson KA, Warner PR. Impaired graft survival in pediatric renal transplant recipients with donor-specific antibodies detected by solid-phase assays. Pediatr Transplantation 2010: 14:730–734. © 2010 John Wiley & Sons A/S. Abstract: SAB assays have increased the sensitivity and specificity to detect HLA alloantibodies, but there is uncertainty about the clinical relevance of SAB-positive alloantibodies when the FCXM is negative. We performed a retrospective study to evaluate the clinical significance of SAB-detected DSA in 82 pediatric recipients of a first kidney transplant between January 2000 and December 2005 who had a negative pretransplant FCXM. Pretransplant sera were evaluated by SAB for DSA. Graft loss and rejection between patients with (DSA+) and without DSA (DSA−) were compared. DSA were detected in 13.9%. Eighty percent of DSA+ subjects were DD transplant recipients vs. 56.9% in the DSA− cohort. The RR of graft loss in DSA+ vs. DSA− was 3.3 (95% CI, 1.4–7.9) and in DD was 4.3 (95% CI 1.4–13.1). By Cox regression, the HR of graft loss in DSA+ vs. DSA− was 2.8 (95% CI 0.7–10.9; p = 0.14) and in DD was 5.1 (95% CI 1–25.6; p = 0.05). Acute rejection occurred in 60% in the DSA+ vs. 44.4% in DSA− (p = 0.5). SAB-detected DSA was associated with impaired renal allograft survival in pediatric renal transplant recipients. Impaired graft survival in pediatric renal transplant recipients with DSA detected by solid-phase assays.