A method has been developed for the identification of unknown brighteners in detergents by a high‐performance liquid chromatography (HPLC)/mass spectrometry (MS)/ultraviolet (UV)/fluorescence system with MS/MS capability. Ten brighteners from five groups—stilbene, biphenyl stilbene, pyrazoline, oxazole, and coumarin derivatives—were separated on a C8 reverse‐phase HPLC column. The UV and fluorescence detectors positively located the brightener peaks and differentiated betweentrans andcis isomers separated by the chromatographic system. Ion‐spray (IS)/MS and IS/MS/MS spectra were then used to identify the structure of unknown brighteners in detergent products. An HPLC with a diode‐array detector provided a quick identification check and accurate quantitative data for known brighteners, even on overlapping peaks.
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Ogura et al. (1995) studied this question.
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