Hierarchically Assembled Two‐dimensional Hybrid Nanointerfaces: A Platform for Bioelectronic Applications

We introduce a hybrid two‐dimensional nanointerface structure for bioelectronic systems. We fabricated smart hierarchically self‐assembled 2D electrobiocatalytic interface system based on the combination of gold nanoparticles doped graphene oxide (GO)‐molybdenum disulfide (MoS2) layered nanohybrid, conjugated with poly (N‐isopropylacrylamide, PNIPAAm) resulting in GO/AuNPs/MoS2/PNIPAAm interface. Horseradish peroxidase (HRP) was subsequently immobilized on the GO/AuNPs/MoS2/PNIPAAm interface through electrostatic interactions giving GO/AuNPs/MoS2/PNIPAAm/Peroxidase electrobiocatalytic interface system as a platform for electrobiocatalysis reactions for biosensing and bioelectronic applications. The electrobiocatalytic activity of the nanohybrid interface structure was studied using hydrogen peroxide (H2O2) as a model analyte. Cyclic Voltammetry showed diffusion controlled electron transfer properties at the interface. The fabricated bioelectrode exhibits a wide linear response to the detection of H2O2 from 1.57 to 11.33 mM, with a detection limit of 3.34 mM (S/N=3) and a capacitance of 8.6 F/cm2.