Expression of chloride channels in mouse skin incisional wounds

This study aimed to determine the types of chloride channels (CLCs) expressed in mouse skin wounds. Twelve male C57BL/6 mice were randomly divided into the skin wound group (SWG) and normal unwounded group (NUG), with 6 mice in each group. A 5-mm lancet wound was made by cutting into the full-thickness skin in the SWG, and the skin was kept intact in the NUG. The expression of CLCs in both groups was evaluated using real-time PCR (RT-PCR) and western blotting (WB). The results of RT-PCR showed that nine CLCs, including CLC-1, CLC-2, CLC-3, CLC-4, CLC-5, CLC-6, CLC-7, CLC-kα, and CLC-kβ, were expressed in both groups. However, the WB results showed that only CLC-2, CLC-7, CLC-kα, and CLC-kβ were expressed in both groups. The results of this study indicate that CLC-2, CLC-7, CLC-kα, and CLC-kβ were expressed in intact and wounded mouse skin according to both RT-PCR and WB analyses.