What question did this study set out to answer?

To investigate the direct effects of luminal reactive nitrogen oxide species on adjacent gastric barrier function using an ex vivo chamber model simulating the gastro-oesophageal junction.

October 26, 2007Gut

Disruption of gastric barrier function by luminal nitrosative stress: a potential chemical insult to the human gastro-oesophageal junction

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Authors

NANobuyuki AraSendai Medical Center KIKatsunori IijimaAkita University KAKiyotaka AsanumaJapan Cancer Society

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Implication

Ex vivo experimental study demonstrates gastric epithelial barrier disruption by luminal reactive nitrogen species in mucosal tissue, suggesting a chemical mechanism for junction injury.

Key Points

To investigate the direct effects of luminal reactive nitrogen oxide species on adjacent gastric barrier function using an ex vivo chamber model simulating the gastro-oesophageal junction.
Mounted rat gastric mucosal membranes in an ex vivo dual-chamber model simulating the microenvironment of the gastro-oesophageal lumen and adjacent mucosa.
Generated luminal reactive nitrogen oxide species (RNOS) through the acidification of physiological concentrations of sodium nitrite.
Assessed epithelial integrity by measuring electrophysiological transmembrane resistance, [3H]mannitol flux, occludin expression, and dinitrosyl dithiolato iron complex diffusion via electron paramagnetic resonance spectroscopy.
Acidified nitrite exposure decreased transmembrane resistance and increased epithelial permeability to [3H]mannitol flux, indicating gastric barrier breakdown.
Nitrosative stress induced derangement of the essential tight junction protein occludin as detected by immunohistochemistry and immunoblotting.
Detection of dinitrosyl dithiolato iron complexes in the tissue confirmed the active diffusion of luminal RNOS into the mucosal barrier.

Cite This Study

Ara et al. (2007) studied this question.

synapsesocial.com/papers/6aa4e613814b48fd3caa2862 https://doi.org/10.1136/gut.2007.128074

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Implication

Ex vivo experimental study demonstrates gastric epithelial barrier disruption by luminal reactive nitrogen species in mucosal tissue, suggesting a chemical mechanism for junction injury.

Disruption of gastric barrier function by luminal nitrosative stress: a potential chemical insult to the human gastro-oesophageal junction

Discussion

Member takes

Implication

Key Points

Cite This Study

Disruption of gastric barrier function by luminal nitrosative stress: a potential chemical insult to the human gastro-oesophageal junction

Discussion

Member takes

Implication

Key Points

Cite This Study

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