E. M. B. Saraiva, P. F. P. Pimenta, M. E. A. Pereira, W. de Souza, Isolation and Purification of Amastigotes of Leishmania mexicana amazonensis by a Gradient of Metrizamide, The Journal of Parasitology, Vol. 69, No. 3 (Jun., 1983), pp. 627-629
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Saraiva et al. (1983) studied this question.