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September 12, 2026Tropical Medicine and Infectious DiseaseOpen Access

Effects of Three Anthelmintics on the Transcriptome of FHs 74 Int Enterocytes

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Authors

KNKornkanok NakudomAGAmornrat Geadkaew-KrencWPWansika Phadungsil

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Overview

In vitro study reveals distinct transcriptional responses to anthelmintics in human enterocytes, suggesting differential host cell pathways are altered during treatment.

Key Points

  • To determine how three common anthelmintic drugs—albendazole, ivermectin, and praziquantel—alter gene expression profiles in human intestinal enterocytes.
  • Cultured human intestinal epithelial cells (FHs 74 Int) were exposed for 24 hours to albendazole (1 µM), ivermectin (15 µM), or praziquantel (1 mM).
  • Cytotoxicity was assessed at 24 and 72 hours, followed by transcriptome profiling using criteria of adjusted p-value (padj) ≤ 0.05 and log2 fold change thresholds.
  • Praziquantel (1 mM) caused negligible cytotoxicity and induced zero significant transcriptional changes at padj ≤ 0.05 and log2 fold change ≥ 0.
  • Albendazole (1 µM) altered 19 genes (17 upregulated, 2 downregulated; padj ≤ 0.05, log2 fold change ≥ 2), modestly upregulating mitotic spindle formation and chromosome segregation pathways.
  • Ivermectin (15 µM) altered 171 genes (106 upregulated, 65 downregulated; padj ≤ 0.05, log2 fold change ≥ 2), downregulating transcription factors linked to proliferation while upregulating metabolic enzymes linked to cell survival.

Cite This Study

Nakudom et al. (2026) studied this question.

synapsesocial.com/papers/6aa51f39327956e4761f97a5https://doi.org/10.3390/tropicalmed11090257
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