Long-lived ratiometric sensors: Luminescent lanthanide complexes are widely used in time-resolved assays of biomolecules, but most of the sensors with these complexes rely on single-point intensity measurements. Herein, we introduce a simple strategy to create ratiometric probes by using salicylic-acid derivatives as the antenna moiety of Tb(3+) complexes. As an example, a probe for alkaline phosphatase (ALP) was developed (see scheme).
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Terai et al. (2012) studied this question.
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