Effects of Dynamin-related Protein 1 Regulated Mitochondrial Dynamic Changes on Invasion and Metastasis of Lung Cancer Cells

Objective: Mitochondrial imbalance of division and fusion will lead to uncontrolled cell growth.This study investigated the effects of mitochondrial dynamics regulated by dynamin-related protein 1 (Drp1) on the invasion and metastasis of lung cancer cells at the cellular level.Methods: Lentivirus-mediated RNAi and gene overexpression vectors containing shDrp1 and Lv-Drp1 were transfected into lung adenocarcinoma cell lines 95D and A549, respectively.An MTT assay was used to assess cell viability and a cell clone assay was used to evaluate the tumorigenic ability of lentivirus-infected cells.Cell invasion and wound healing assays were used to assess cell invasiveness and the migration rate after lentivirus infection.Annexin V-APC staining was used to determine the cell apoptosis rate.Results: In 95D cells, when the Drp1 gene is overexpressed (OE) the proliferation rate and apoptosis rate were significantly higher than those in the control group (NCOE) (P < 0. 05).There was no significant difference in clone number, invasion rate, and migration rate between the two groups (P > 0. 05).The proliferation rate and clone number in the shDrp1 infected 95D cell group (KD) were significantly lower than those in the control group (NCKD) (P < 0. 05).There was no difference in apoptosis rate, invasion rate, and migration rate between h (P > 0.05).In A549 cells, unlike in 95D cells, the invasion rate of the KD group was 25% lower than that of the NCKD group (P < 0.05).After 8 hours, the cell migration rates of the two groups were basically the same, but after 24 hours, the migration rate of the KD group was 10% lower than that of the NCKD group (P < 0.05).Compared with the NCOE group, the migration rate of the OE group increased significantly (P < 0.05).Conclusion: Mitochondrial Drp1 is associated with the proliferation, invasion, and metastasis of lung adenocarcinoma cells.Inhibition of Drp1 expression may contribute to anti-tumor therapy for lung cancer.