Key result
Exchanging segment A noncoding regions in IBDV chimeras does not determine viral pathotype differences.
The noncoding regions of segment A in infectious bursal disease virus are not responsible for the differing pathotypes between serotypes I and II.
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Excludes segment A noncoding regions as IBDV pathotype determinants; leaves open identification of responsible elements in other genomic regions.
Two serotypes, I and II, have been identified for infectious bursal disease virus (IBDV), a member of the family BIRNAVIRIDAE: Here, the generation by reverse genetics of IBDV chimeras in segment A of the bisegmented genome is reported. The 5- and 3'-noncoding regions (NCRs) of a serotype II strain were exchanged with the NCRs of a full-length cDNA clone of segment A of a serotype I strain. Isolated chimeric viruses were characterized in cell culture and susceptible chickens. The results show that IBDV chimeras in segment A were able to replicate in cell culture and that VP1 encoded by a serotype I segment B is functionally active with serotype I NCRs as well as with serotype II NCRs. Chimeric viruses infected susceptible chickens and caused mild depletion of bursal cells. Thus, the noncoding regions of segment A are not responsible for the different pathotypes of IBDV serotypes I and II.
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Schröder et al. (2000) studied Infectious bursal disease virus (IBDV) infection. IBDV chimeras in segment A (exchanged noncoding regions) was evaluated on Viral replication in cell culture and pathogenicity in susceptible chickens. IBDV chimeras with exchanged noncoding regions in segment A were viable, replicated in cell culture, and caused mild depletion of bursal cells in chickens, indicating these regions do not determine pathotype differences.
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