Key result
Dual-contrast preparation improves 3D magnetic resonance microscopy image quality in chick embryos versus previous methods.
A novel dual-contrast preparation method for chick embryos significantly improves 3D magnetic resonance microscopy image quality for studying cardiovascular development.
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May advance preclinical imaging of embryonic cardiovascular development; leaves open translation to human applications.
Previous work demonstrated the power of three-dimensional (3D) magnetic resonance microscopy (MRM) to follow complicated morphologic development in the embryonic cardiovascular system. In this study we describe a new dual-contrast method for specimen preparation that combines perfusion fixation and immersion in fixative with macro- and small molecular gadolinium agents to provide enhanced definition of both the heart wall and chamber. MRM was performed at 9.4 T with image resolutions of 25, 31, and 50 microm isotropic voxels for three stages of chick embryos (day 4, day 5.5, and day 9), and compared to histological sections of the same embryos. The results show considerable improvement of image quality over previous efforts, with better signal-to-noise ratio (SNR) and contrast between the cardiac chamber and myocardial wall. Excellent correlation was shown between the MRM images and histological sections. Thus, 3D high-resolution MRM in combination with the dual-contrast technique is useful for acquiring quantitative 3D morphologic data regarding heart development.
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Zhang et al. (2003) studied Chick embryonic cardiovascular system development. Dual-contrast method for specimen preparation vs. Previous efforts and histological sections was evaluated on Image quality, signal-to-noise ratio, and contrast. A new dual-contrast specimen preparation method for 3D magnetic resonance microscopy improved image quality, signal-to-noise ratio, and contrast in chick embryos compared to previous efforts.
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