Stereochemistry of oxidation of benzylamine by the amine oxidase from beef plasma

2.1. Enzyme and substrates The enzyme was purified to homogeneity by a modification of method in [l] and will be detailed in [2]. The enzyme was stored as a suspension in 0.1 M Na2HP04/KH2P04 (pH 7.0) buffer containing ammonium sulphate to 0.55 saturation. Purified enzyme had spec. act. 0.36 units/mg protein; 1 unit catalyses the formation of 1 pmol benzaldehyde (e2s0 = 1.28 X lo4 M-’ cm-‘) per minute at 25’C under the standard assay conditions [ 31. (KS)-, (II)and (S)-[methylene-3Hl]benzylamines were synthesised as in [4]. Benzylamine samples were assayed using ninhydrin [ 51. Liver alcohol dehydrogenase, catalase and NADH were obtained from Sigma.