Five‐Component Cascade Synthesis of Nucleotide Analogues in an Engineered Self‐Immobilized Enzyme Aggregate

Pathway in a particle: A five-enzyme biosynthetic pathway was self-immobilized to form a single biocatalyst for generation of purine nucleotide analogues from D-ribose. This method provides an alternative to engineering biosynthetic pathways in whole cells that obviates problems associated with toxicity, transport and genetic regulation.