Production of new class-specific polyclonal antibody for determination of fluoroquinolones antibiotics by indirect competitive ELISA

A class-specific polyclonal antibody against fluoroquinolones was obtained by immunising rabbits with a novel designed hapten, which preserved the essential group of fluoroquinolones. With this specific polyclonal antibody, 13 fluoroquinolones antibiotics were simultaneously detected in indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). The established ic-ELISA was class-specific to detect 13 fluoroquinolones such as norfloxacin, ciprofloxacin, ofloxacin etc., and the limit of detection (LOD) desirable was (0.01–10 ng/ml) and the acceptable recovery range was between 62 and 95%. Results proved that this group-specific ic-ELISA method was reliable and suitable for multi-residue determinations of 13 fluoroquinolones.