Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization

DNA inserts obtained from a cloning of the penae~d shrimp baculovirus, BP, were nonradioactively labelled with digoxigenin for use as gene probes to detect BP in paraffin-embedded, formalin/alcohol-fixed tissue sections using in sit11 hybridization.Results show that the probes detect BP in various species of shrimp from different geoglaphicdl dreas.The probes demonstrate no reaction to BP-negative tissue sections.The probes also demonstrate no reaction to tissue infected with other penaeid shrimp viruses.