Methodological study demonstrates accurate stereoscopic calibration using cylindrical polymer monofilaments in electron microscopy, highlighting primary sources of stereo-image distortion.
SUMMARY In order to obtain accurate measurements of the topography of surfaces using the scanning electron microscope some form of stereoscopy is generally necessitated. One technique has already been described elsewhere (Unsworth & Hepworth, 1971) along with a detailed calibration procedure using polymer monofilaments as standard specimens. Because of the special case they represent, the present paper justifies the use of such specimens (with an almost perfect circular cross‐section) on both theoretical and practical grounds. Further, since the monofilaments possess a known geometry, conclusions are possible regarding some sources of distortion of the stereo‐image.
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Hepworth et al. (1973) studied this question.
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