Schistosome infectivity in the snail, Biomphalaria glabrata, is partially dependent on the expression of Grctm6, a Guadeloupe Resistance Complex protein.

Schistosomiasis is one of the most important neglected tropical diseases.Despite effective chemotherapeutic treatments, this disease continues to afflict hundreds of millions of people.Understanding the natural intermediate snail hosts of schistosome parasites is vital to the suppression of this disease.A recently identified genomic region in Caribbean Biomphalaria glabrata snails strongly influences their resistance to infection by Schistosoma mansoni.This region contains novel genes having structural similarity to known pathogen recognition proteins.Here we elaborate on the probable structure and role of one of these genes, grctm6.We characterised the expression of Grctm6 in a population of Caribbean snails, and performed a siRNA knockdown of Grctm6.We show that this protein is not only expressed in B. glabrata hemolymph, but that it also has a role in modulating the number of S. mansoni cercariae released by infected snails, making it a possible target for the biological control of schistosomiasis. Author summarySchistosomiasis is one of the most prevalent parasitic diseases in the world.Though treatments for schistosomiasis infection exist, there is no vaccine, and reinfection is common in areas where the parasite occurs.One possible way to mitigate schistosomiasis is by controlling the transmission of the parasite larvae from the snails that carry them.Understanding the snail-parasite relationship is essential for the development of new means to interrupt transmission of the parasite from snails to humans.Snails possess immune mechanisms for fighting infection, most of which are based in hemolymph tissue.Here we characterize a novel protein, Grctm6, in a snail host of schistosome parasites.Grctm6 is structurally similar to certain other immune proteins and is present in snail hemolymph.Importantly, we demonstrate that the release of schistosomes by infected snails is exacerbated when this protein is experimentally suppressed in live snails.These results