Identification and differentiation of different species of microorganisms were explored through spectroscopy. Themid-infrared (MIR) and near-infrared (NIR) spectral profiles of five different microorganisms could be classified into separategroups when spectral data were compressed using principal component analysis (PCA) and processed by canonical variateanalysis (CVA). Fourier transform mid-infrared (FT-MIR) spectroscopy was found to be a rapid method for identificationand differentiation of not only closely related Escherichia coli strains but also pathogenic and non-pathogenic E. coli strains.Results clearly demonstrate the potential of FT-MIR spectroscopy as a tool for microbial strain identification andclassification. The method is rapid, inexpensive, reproducible, and requires minimum sample preparation.
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Sivakesava et al. (2004) studied this question.