High-performance liquid chromatography (HPLC) in combination with atmospheric pressure chemical ionization mass spectrometry (APcI-MS) was applied to the determination of the phenolic fraction found in methanolic extracts of sunflower seeds (mainly chlorogenic acid and derived compounds). These extracts were directly separated by HPLC and detected by both negative and positive APcI-MS. Abundant structural information about these compounds can be obtained even at low extraction cone voltages. This method has been shown to be a rapid and effective method for the analysis of crude extracts from sunflower seeds.
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Aramend�a et al. (2000) studied this question.
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