Determination of GS-441524 in The Polyethylene Glycol Preparation by HPLC Coupled with High Resolution Mass Spectrometry

GS-441524, a nucleoside analogue molecule, is both intermediate and metabolite of remdesivir.Because of proved effectiveness in animals, GS-441524 is expected to be a better potential drug than remdesivir in the treatment of Covid-19.Since GS-441524 is not easily soluble in pure water, polyethylene glycol (PEG) was used as the auxiliary solvent.The developed LC-MS method utilized a UHPLC coupled with a TOF mass spectrometer and developed a gradient elution to efficiently separate GS-441524 and other PEG-400 molecules.The chromatographic separation completed within 6 minutes.GS-441524 was identified in peak at 0.9 min explicitly.No interferent was co-eluted.UV detection was not proved suitable for the determination of GS-441524 under this condition.The correlation coefficient R 2 (0.9970) indicated a good linear relationship in range of 0.1 ~ 10 μg/mL.The LOD and LOQ of the method was 0.05μg/mL and 0.1 μg/mL respectively.A concrete sample was accuracy analyzed.The developed LC-MS method provided a simple, rapid, accurate and precise analysis protocol for the determination of GS-441524 in preparation system with PEG-400 and water as solvent.