What is the clinical evidence from this study?

Study design: Other. Intervention: Site-directed isotope labeling and ATR-FTIR spectroscopy. Primary outcome: Local secondary structure and orientation of the transmembrane domain of phospholamban.

What does this research mean for the field?

The transmembrane domain of phospholamban is predominantly alpha-helical, embedded within the lipid bilayer with an axial tilt of approximately 30 degrees relative to the membrane normal. Novelty: ClaimNovelty.CONFIRMATORY. Consensus alignment: ConsensusAlignment.SUPPORTS_CONSENSUS.

April 1, 1996Biophysical JournalOpen Access

Fourier transform infrared spectroscopy and site-directed isotope labeling as a probe of local secondary structure in the transmembrane domain of phospholamban

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Key result

ATR-FTIR spectroscopy and isotope labeling reveal the phospholamban transmembrane domain is predominantly alpha-helical and lipid-embedded.

Population

Hydrophobic C-terminal 28 amino acid fragment of phospholamban reconstituted into…

Design

Preclinical

Authors

CLCheryl F.C. LudlamBoston University IAIsaiah T. ArkinDepartment of Health XLX M Liu

Discussion

Member takes

Implication

Refines phospholamban membrane topology models; extends biophysical data but leaves open clinical translation to heart failure.

Structured PICO

Population

Hydrophobic C-terminal 28 amino acid fragment of phospholamban (hPLB) reconstituted into dimyristoylphosphatidycholine bilayer membranes

Intervention

Polarized attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy and site-directed isotope labeling

Outcome

Local secondary structure and transmembrane orientation of hPLBsurrogate

ATR-FTIR spectroscopy and isotope labeling confirm that the transmembrane domain of phospholamban forms an alpha-helical structure embedded in the lipid bilayer.

Cite This Study

Ludlam et al. (1996) studied this question. Site-directed isotope labeling and ATR-FTIR spectroscopy was evaluated on Local secondary structure and orientation of the transmembrane domain of phospholamban. ATR-FTIR spectroscopy and site-directed isotope labeling demonstrated that the transmembrane domain of phospholamban is predominantly alpha-helical and embedded in the lipid bilayer.

synapsesocial.com/papers/6aa5b6026a6f69d61978ec88 https://doi.org/10.1016/s0006-3495(96)79735-7

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Fourier transform infrared spectroscopy and site-directed isotope labeling as a probe of local secondary structure in the transmembrane domain of phospholamban

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Fourier transform infrared spectroscopy and site-directed isotope labeling as a probe of local secondary structure in the transmembrane domain of phospholamban

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider