Chemopreventive effects of elm tree bark extract onHelicobacter pylori‐associated mouse gastric carcinogenesis

Background and aim:Extracts of elm tree (Ulmus davidiana japonica) have long been used for the treatment of inflammatory diseases in traditional medicine. Severalin vitrostudies have indicated that some components of the elm tree have anti‐inflammatory and/or anti‐oxidative effects. We investigated the chemopreventive effects of bark extract of elm tree (BEE) on a chronic inflammation‐associated mouse gastric cancer model.Methods:Gastric cancer was generated by the combination treatment ofN‐methyl‐N‐nitrosourea andHelicobacter pylorito C57BL/6 mice. Various concentrations of BEE (0, 125, 250, 500, and 1,000 ppm) was fed to the mice for 38 weeks.Results:The incidence of gastric tumors at 50 weeks were significantly lower in the highest dose BEE‐fed mice (26.67%) than control mice (85.71%) (P < 0.01). The multiplicity and size of tumors were also significantly lowered by BEE feeding in a dose‐dependent manner (P < 0.01). In addition, BEE decreased the nuclear β‐catenin localization and nuclear cyclin‐D1 expression in adenocarcinomas. Furthermore, BEE suppressedH. pylori‐associated chronic inflammation determined by histologic gradings ofH. pyloridensity, chronic gastritis, glandular atrophy and intestinal metaplasia in non‐tumorous gastric mucosae.Conclusions:These findings suggest that BEE inhibits the development of gastric tumorigenesis via suppression ofH. pyloriinfection‐associated chronic inflammation.