The methanolic extract of fruit bodies of cultivated Ganoderma lucidum was separated by silica gel column chromatography and preparative thin‐layer chromatography to give ten compounds. On the basis of spectral analysis, chemical procedures and gas chromatography, d‐mannitol (1), ergosta‐7, 22‐dien‐3β‐yl palmitate (2), ergosterol (3), ergosta‐7, 22‐dien‐3β‐ol (4), 5α‐lanosta‐7,9(11),24‐trien‐3β,26‐diol (5), ergosterol peroxide (6), 24,25,26‐trihydroxy‐5α‐lanosta‐7,9(11)‐dien‐3‐one (7), 5α‐lanosta‐7,9(11)‐dien‐3β,24,25,26‐tetraol (8) and 8,9‐epoxyergosta‐5,22‐dien‐3β,15‐diol (9) were identified. Among these compounds, 8,9‐epoxyergosta‐5,22‐dien‐3β,15‐diol was first separated from Ganoderma lucidum.
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Chiang et al. (1991) studied this question.
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