Studies on the galactomannan-degrading enzymes produced bySporotrichum cellulophilum

Extracellular mannanase activity produced by Sporotrichum cellulophilum was purified into two components using acetone precipitation, SP-Sephadex C50 ion exchange chromatography and preparative polyacrylamide gel electrophoresis.The purified mannanse components, M1 and M2, had molecular weights of 108000-112000 and 32200-36000 respectively.Component M 1 was shown to contain 2 subunits having molecular weights of 62000 and 50000.M1 and M2 had similar pH-activity profiles with pH optima of 5.5 and 6.0 respectively.M1 was more thermostable than M2: half lives of the enzymes at 70 ~ were 30 and 9 min for M1 and M2 respectively.