Prunetin Relaxed Isolated Rat Aortic Rings by Blocking Calcium Channels

Figures

• 

  Figure 1. The concentration-response relaxation effect of prunetin on aortic rings pre-contracted by phenylephrine (PE, 1 μM) (A) with [(E+)] or without [(E−)] vascular endothelium; (B) Representative traces under the indicated conditions. Values are expressed as mean ± standard error of mean (SEM) (n = 4). ** p < 0.01, ## p < 0.01 vs. control.

• 

  Figure 2. The inhibitory effect of prunetin (1–10 μg/mL) on the contraction induced by CaCl2 on endothelium-denuded aortic rings pre-contracted by phenylephrine (PE, 1 μM) (A,C) or KCl (60 mM) (B,D) in the presence or absence (control) of prunetin. (C) and (D) are representative traces under the indicated conditions. Values are expressed as mean ± SEM (n = 4–6). * p < 0.05, ** p < 0.01 vs. control.

• 

  Figure 3. The vasorelaxant effect of prunetin (1–30 μg/mL) on vessels pre-contracted by phenylephrine (PE, 1 μM) with or without pre-treatment with 2-aminoethoxydiphenyl borinate (blocker of store-operated Ca2+ entry, 2-APB) (A,B) and the inhibitory effect of prunetin (10 μg/mL) on vasocontraction induced by caffeine (RyR agonist, 10 mM) (C,D). (B,D) are representative traces under the indicated conditions. Values are expressed as mean ± SEM (n = 4–5). * p < 0.05 vs. control.

• 

  Figure 4. Concentration-response relaxation effect (A) and representative traces of the effect (B) of prunetin on endothelium-denuded aortic rings pre-contracted by phenylephrine (PE, 1 μM) in the presence or absence (control) of Y-27632 (Rho-kinase inhibitor, 1 μM). Values are expressed as mean ± SEM (n = 4).

• 

  Figure 5. Concentration-response relaxation effect (A) and representative traces of the effect (B) of prunetin on endothelium-intact aortic rings pre-contracted by phenylephrine (PE, 1 μM) with or without (control) glibenclamide (KATP channel blocker, 10 μM), 4-aminopyridine (KV channel blocker, 4-AP, 1 mM), or tetraethylammonium (BKCa channel blocker, TEA, 5 mM). Values are expressed as mean ± SEM (n = 4–6).

• 

  Figure 6. Hypotensive effect of prunetin on the changes in systolic blood pressure (SBP) of SHR. SBP was measured by using the non-invasive tail cuff system. The values are expressed as the mean ± SEM (n = 6). * p < 0.05, ** p < 0.01 vs. control.