Potential of Bacillus subtilis to produce acidic protease under mutagenic condition

Protease from Bacillus subtilis has wide applications in industrial sectors.A total of 28 bacteria were isolated from marine mud sample, of which 3 isolates exhibited constructive results for protease production in skim milk, gelatin and casein agar. Among three isolates, MMK 2/8 had superlative enzymatic activity in skim milk broth (170 U/mL) and was subjected for taxonomical identification, which was found to be Bacillus subtilis. The finest conditions were found to be at pH 6.0, temperature 40 C and 1% NaCl concentration and their maximal enzyme activitywas 354 U/mL, 437 U/mL and 263U/mL respectively, at 36 hrs of incubation. The molecular weight of the protease enzyme was considered to be approximately 50 kDa by SDS-PAGE. The enzyme activity was enriched by exposing it to ultra violet radiation [UV] for 60 secand it was observed to be 610 U/mL. Hence, we charted that the production of acidic protease treated with ultra violet radiation was maximum when compared with the parental strain.