Intracellular adenosine triphosphate and cyclic adenosine 3′,5′-monophosphate concentrations during derepression of actinomycin biosynthesis

Measurements of adenosine triphosphate and of cyclic adenosine 3′,5′-monophosphate in the mycelium of Streptomyces antibioticus during growth in a medium containing a mixture of glucose and galactose showed no marked changes in concentration during the period when glucose was exhausted and the synthesis of actinomycin began. Thus, these nucleotides do not appear to have a role in mediating catabolite repression of either carbon source utilization or antibiotic production in this organism. Since no NADP-glucohydrolase activity was detected in cell extracts, this enzyme is also excluded from apossible role in regulating actinomycin biosynthesis.