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Starch gel electrophoresis in three buffers was used to assay 21 enzyme systems in the two butterfly species Aricia agestis and A. artaxerxes. 16 enzymes showed activity when stained for, but no absolute interspecific difference was found in the patterns. Methods for a statistical, interpopulational comparison of multiple allelic systems have been discussed. On basis of allozyme variation in three enzymes it has been shown that the two species are very close sibling species.
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Jens Erik Jelnes (2009) studied this question.
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