A simple two-step procedure is described for the concentration and purification of hepatitis B antigen. The antigen is precipitated by polyethylene glycol and then adsorbed on and eluted from an insoluble polyelectrolyte, polyelectrolyte 60, which is a cross-linked copolymer of isobutylene maleic anhydride.
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Rizzo et al. (1972) studied this question.
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