The influence of processing protocol on the ultrastructure of bean leaf cells

Bean leaf cells exhibit ultrastructural damage when glutaraldehyde fixatives or buffer washes are applied for prolonged periods. The maximum times in these processing steps are influenced by the choice of buffer. Prolonged periods in low concentrations of acetone also cause damage. This damage is progressively less obvious in higher concentrations. Extended holding times In anhydrous acetone are possible, especially if phosphate or cacodylate buffers are employed during fixation. Selle van boontjieblare vertoon ultrastrukturele skade indien dit vir lang tye aan fikseermiddels of aan buffer-was oplossings blootgestel word. Die maksimum periodes in hierdie prosesseerstappe word beïnvloed deur die keuse van buffer. Lang periodes in lae konsentrasies asetoon veroorsaak ook skade. Hierdie skade word progressief minder in hoër konsentrasies. Lang periodes in anhidriese asetoon is moontlik, veral indien fosfaat- of kakodilaat- buffers gedurende fiksering gebruik word.