An Analysis of the Control of Phosphorylation-Coupled Respiration in Isolated Plant Mitochondria

The control of phosphorylation-coupled respiration in isolated turnip (Brassica rapa) mitochondria was investigated according to the principles of metabolic control analysis as developed by H. Kacser and J. A. Burns ([1973] Symp Soc Exp Biol 32: 65-104) and R. Heinrich and T. A. Rapoport ([1974] Eur J Biochem 42: 97-105). Inhibitor titration studies were used to determine quantitatively the amount of control exerted by four individual processes-cytochrome bc(1), cytochrome oxidase, H(+)-ATPase, and the adenine nucleotide carrier-on respiratory flux under ADP-excess (state 3) and ADP-limited (state 4) conditions with a range of respiratory substrates. Under state 3 conditions control strength was found to be distributed between cytochrome oxidase, cytochrome bc(1), and H(+)-ATPase in decreasing order of importance. The adenine nucleotide carrier exerted no control on respiratory flux under these conditions. Control strength at each step was found to vary with different substrates and with the respiratory flux as altered by ADP supply, i.e. virtually zero control strength at cytochrome oxidase and cytochrome bc(1) under state 4 conditions.