A theoretical study of concentration‐focusing in electrophoresis is presented. The term is defined and the model of calculation discussed. Starting with a system of partial differential equations obtained by conservation of mass, several expressions are derived, including: nonstationary electrophoresis with complex‐formation; diffusion‐restricted and rate‐of‐reaction‐restricted stationary focusing. The influence of process parameters on the line‐width of a focused substance is investigated. It is possible to account for the observed effects caused by the electric field‐strength, the gradient of the complex forming agent, the diffusion, and the rate of reaction. Separation by focusing as compared with ordinary electromigration is characterized by short duration and insensitivity towards perturbations.
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Ernst Schumacher (1957) studied this question.
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