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May 25, 2009The Journal of Cell Biology75 citationsOpen Access

Rescue of skeletal muscle α-actin–null mice by cardiac (fetal) α-actin

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Why the study?

Does cardiac alpha-actin (ACTC) expression rescue survival and muscle function in Acta1 knockout mice?

Population

Acta1 knockout mice (which normally die by 9 days after birth)

Comparison

Expression of cardiac alpha-actin via transgenic… vs Acta1 knockout mice without ACTC transgene

Design

Preclinical

Follow-up

to old age

Key result

Transgenic expression of cardiac alpha-actin (ACTC) rescued Acta1 knockout mice from early postnatal death, enabling survival to old age without impairment in skeletal muscle function.

Authors

GRGianina RavenscroftCJConnie JackamanAFAleksandra Filipovska

Discussion

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Overview

Extends evidence for actin isoform interchangeability; leaves open therapeutic translation to human ACTA1 myopathies.

Structured PICO

Does cardiac alpha-actin (ACTC) expression rescue survival and muscle function in Acta1 knockout mice?

P
Population
Acta1 knockout mice (which normally die by 9 days after birth) crossed with ACTC transgenic mouse lines to evaluate if ACTC can replace ACTA1 in postnatal skeletal muscle.
I
Intervention
Expression of cardiac alpha-actin (ACTC) via transgenic cross (high expressing line)
C
Comparator
Acta1 knockout mice without ACTC transgene (implied, die by 9 days after birth)
O
Outcome
Survival to old age and skeletal muscle function (grip strength, rotarod, locomotor activity)hard clinical

Cardiac alpha-actin (ACTC) can functionally replace skeletal muscle alpha-actin (ACTA1) in postnatal skeletal muscle, suggesting ACTC reactivation as a potential therapy for ACTA1 congenital myopathies.

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Cite This Study

Ravenscroft et al. (2009) studied ACTA1 congenital myopathies. Cardiac alpha-actin (ACTC) transgene vs. Acta1 knockout mice (expected to die by 9 days after birth) was evaluated on Survival and skeletal muscle function (grip strength, rotarod, locomotor activity). Transgenic expression of cardiac alpha-actin (ACTC) rescued Acta1 knockout mice from early postnatal death, enabling survival to old age without impairment in skeletal muscle function.

synapsesocial.com/papers/6aa67fdc014c9b816df4f18fhttps://doi.org/10.1083/jcb.200812132
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