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A reversed-phase high-performance liquid chromatography (HPLC) method for the determination of sterols in sea urchin (Paracentrotus lividus) gonads is described. After saponification with KOH, samples are extracted with hexane, evaporated to dryness, and dissolved in HPLC eluant. Sterols were separated using a C18 column as the stationary phase, methanol-acetonitrile (30:70, v/v) as the mobile phase, and ultraviolet detection at 205 nm. Three sterols were identified and quantitated: desmosterol, fucosterol, and cholesterol. Relative standard deviations (%) were 2.32 for desmosterol, 2.31 for fucosterol, and 2.13 for cholesterol. Limits of detection were 2.31, 0.56, and 2.33 µg/mL for desmosterol, fucosterol, and cholesterol, respectively.
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López‐Hernández et al. (1999) studied this question.
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