Evaluation of the Hybrid Capture human papillomavirus deoxyribonucleic acid detection test

OBJECTIVE: Our purpose was to evaluate the sensitivity and accuracy of a new, nonradioactive human papillomavirus deoxyribonucleic acid detection method.STUDY DESIGN: Cervical samples from 520 women were assayed for human papillomavirus deoxyribonucleic acid with both the Hybrid Capture test and polymerase chain reaction.RESULTS: Human papillomavirus deoxyribonucleic acid was detected with Hybrid Capture in 106 (42%) of 254 samples from women with no evidence of cervical intraepithelial neoplasia and 211 (79%) of 266 with cervical intraepithelial lesions or cervical cancer.There was a good correlation between Hybrid Capture and polymerase chain reaction.Hybrid Capture correctly identified 92% of samples found to contain a human papillomavirus type with a high or intermediate oncogenic risk with polymerese chain reaction.Although Hybrid Capture can quantify the amount of human papillomavirus deoxyribonucleic acid present in a sample, no correlation was observed between the relative amount of human papillomavirus deoxyribonucleic acid detected with Hybrid Capture and the grade of cervical lesion.CONCLUSION: The Hybrid Capture test is a sensitive and accurate method for identifying human papi,omavirus types of high and intermediate oncogenic risk in clinical specimens.(AM d Oesl~r G~ECOL 1995;173:1432-7.)Key words: Human papillomavirus, deoxyribonucleic acid, Hybrid Capture test, cervical cancer, polymerase chain reaction It is now almost universally accepted that the majority of invasive cervical cancers and high-grade cervical intraepithelial neoplasia are associated with human papiUomavirus (HPV) types that have specific high and intermediate oncogenic risk) -5 Because of this, there has been increasing interest in using HPV deoxyribo-From the Department of Pathology, ~ the Department of Obstetrics and b c Gynecology, and the Department of