In Riccia crystallina only a few spores germinated after 3-4 months in 4-5% of the cultures attempted. Cold treatment (8-150C) shortened the dormancy period to 15 days and increased the percentage of germination. In inducing the reproductive phase, temperature again turned out to be the critical factor. Day length did not seem to have much effect so long as a certain mini- mum period of illumination was provided. Fertilization took place in the normal way, and mature sporophytes were produced in vitro.
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Chopra et al. (1973) studied this question.
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