Key result
Rat myocardial Nanog protein expression peaks embryonically and nearly vanishes by postnatal day 30.
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Animal data only; leaves open Nanog as a target for cardiac regeneration research.
OBJECTIVES: To investigate the expression dynamic of nanog gene in the development of rat myocardial tissues. METHODS: SD rats were studied at 5 time points before and after birth. The techniques of immunohistochemistry, immunofluorescence, western blotting and RT-PCR were used to investigate the expression of nanog gene in the rat myocardial tissues at different embryonic (E) and postnatal (P) stages, and image analysis system was used for the quantitative analysis. RESULTS: The immunohistochemistry, immunofluorescence and western blotting analyses have shown that expression of nanog protein was highest in the rat myocardial tissues at E18, then it gradually declined at postnatal stages (P<0.05), and became nearly undetectable in most myocardial tissues at P30 with very few remaining nanog-positive cells. RT-PCR result indicated that the expression of nanog gene was strong at E18, but gradually decreased from E18 to P30. CONCLUSION: The mRNA transcription and protein translation of nanog gene in the rat heart gradually decreased with every consecutive growth stage. This indicates that nanog gene has potential regulatory functions in the differentiation of myocardial cells during rat development.
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Guo et al. (2015) studied Developing rat myocardial tissues. Developmental stage (embryonic vs postnatal) vs. Different time points was evaluated on Expression dynamic of nanog gene (p=<0.05). Nanog protein expression was highest in rat myocardial tissues at embryonic day 18 and gradually declined at postnatal stages (P<0.05), becoming nearly undetectable by postnatal day 30.
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