In this study, an effective enrichment technique was applied to isolate different bacterial strains with capabilities to utilize dibenzothiophene (DBT) as a model compound of polyaromatic sulfur heterocyclic compounds (PASHs). Twenty-eight different desulfurizing bacterial strains were isolated from a mineral coke sample with sulfur content of 3.8%. Only nine of them showed the ability to utilize DBT as a sole-sulfur source through the 4S-pathway without altering its hydrocarbon skeleton. From all; a Gram +ve bacterial isolate designated C19 showed a higher biodesulfurization (BDS) efficiency relevant to the well-known biodesulfurizing bacterium strain R. erythropolis IGTS8, recording 66.85% and 50% removal of 1000 ppm DBT with the production of 31.98 and 31.34 ppm 2-hydroxybiphenyl (2-HBP), as a dead end product, respectively. C19 was identified by 16S rDNA gene sequence analysis to be Brevibacillus invocatus C19 (NCBI Gene Bank Accession no. KC999852) with similarity of 99.05%. BDS of different PASHs was also studied and Brevibacillus invocatus C19 showed good BDS capabilities compared to those of IGTS8.
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Nassar et al. (2013) studied this question.