The effects of castration and testosterone treatment on the activity of hexokinase isozymes and their distribution between soluble and particulate fractions in the male sex accessory glands were investigated. The activities of several other soluble enzymes were also delineated. Testosterone treatment had little or no effect in 24 hours on the concentration in rat prostate cytosol of the enzymes measured, other than hexokinase. Hexokinase concentration decreased to 70% of control value on the first day, to 54% on the second day after castration. Testosterone injected on the first day after castration caused a 2.3 fold increase in hexokinase concentration. Actinomycin D (24 μg/100 g) or cycloheximide (60 μg/100 g) prevented the increase in hexokinase concentration induced by testosterone. Starch gel electrophoresis and thermostability experiments revealed that type II is the predominant isozyme of hexokinase in the ventral prostate whereas type I predominates in the seminal vesicle. The activity of type II (but not that of type I) appears to be under the control of androgens. (Endocrinology89: 1162, 1971)
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Santti et al. (1971) studied this question.