Adenyl cyclase activity of chick oviduct homogenates after various conditions of in vivo hormonal stimulation was assayed by measuring the conversion of alpha 32P-ATP to 32P-cyclic 3′,5′-AMP. Oviducts from chicks injected with diethylstilbestrol (DES) showed no immediate change in adenyl cyclase activity, nor was there an activation with chronic DES administration. However, if chicks were given a single dose of progesterone, a delayed activation of adenyl cyclase was noted at 3–6 hr, and reached 250% of control by 24 hr. Enzyme activity at maximal progesterone stimulation was 50% of “total” adenyl cyclase activity as estimated with NaF. These studies suggest that 3′,5′-cyclic AMP does not play a primary role in response of the oviduct to DES, but may play a secondary role in the induction of avidin synthesis by progesterone. (Endocrinology86:1019, 1970)
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Kissel et al. (1970) studied this question.