The fertile egg method of D'arcy, et al. was found to be suitable for anti-inflammatory screening of extracts of natural sources. The suitable primary incubation period and implantation period were 9 and 4 days, respectively. Certain dose of steroidal anti-inflammatory drugs, e.g., 6.3 μg/disc of dexamethazone, significantly inhibited the granulation tissue formation of chorio-allantoic membrane in the established method. Non-steroidal drugs, such as phenylbutazone, showed inhibitory action at 500 μg/disc. This screening method was applied to aqueous methanolic (1 : 1) extracts from more than a thousand crude drugs and plants and 62 extracts were found to be active at 800 μg/disc. It was found that berberine-containing plants, e.g., coptis rhizome, were strongly active even in a dose of 400 μg/disc.
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Otsuka et al. (1974) studied this question.