SALL4 is a novel therapeutic target in intrahepatic cholangiocarcinoma

// Gang Deng 1 , Lei Zhu 1 , Feizhou Huang 1 , Wanpin Nie 1 , Wei Huang 1 , Hongbo Xu 1 , Shaopeng Zheng 1 , Zhongjie Yi 1 , Tao Wan 1 1 Department of Hepatobiliary and Pancreatic Surgery, the Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China Correspondence to: Feizhou Huang, e-mail: feizhou_huang@163.com Keywords: intrahepatic cholangiocarcinoma, SALL4, proliferation, migration, invasion Received: May 07, 2015 Accepted: July 27, 2015 Published: August 07, 2015 ABSTRACT Intrahepatic cholangiocarcinoma (ICC) is the most common and deadly disease of the biliary tree due to its poor prognosis. Sal-like protein 4 (SALL4), a stem cell marker, has been identified as a potential target for aggressive hepatocellular carcinoma (HCC). In our study, 175 ICC cases with an average age of 55 years were included, and 53% (93/175) were male. And 28 adjacent non-tumor tissues were also collected. The SALL4-positive immunoreactivity was detected in a total of 102 ICC cases (58%), whereas all 28 adjacent tissues showed negative staining. Univariate analysis, showed that the SALL4-positive ICC cases had significantly more frequent lymph nodal metastasis ( P = 0.0460), vascular invasion ( P < 0.0001), and nerve invasion ( P < 0.0001). Furthermore, the strong SALL4-positive cases ( n = 7, 5 months) had shorter overall survival, when compared to moderate SALL4-positive ( n = 46, 9 months) or SALL4-negative cases ( n = 73, 7 months), respectively. Our data also suggest that SALL4 may be involved in the regulation of epithelial-mesenchymal transition (EMT) in ICC. Those results for the first time indicate an oncogenic role of SALL4 in ICC. Therefore, SALL4 may serve as a promising therapeutic target for ICC.