Investigation of Antimicrobial Fatty Acids from Medicinal Artist Conk Mushroom Ganoderma applanatum (Pers.) Pat. (Aphyllophoromycetideae) by TLC and Spectroscopic Detection

In this study, we have investigated methanol extractions of different layers (cutis, context, and tube) of Ganoderma applanatum (artist conk mushroom) fruiting bodies to evaluate quantity and structurally elucidate antibacterial compounds. The extractions and quantity evaluations of bioactive compounds have been performed by using high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). Mass and 1H NMR spectroscopy techniques have been used for structural determinations. We identified antibacterial compounds belonging to saturated fatty acids and determined that palmitic acid (hexadecanoic acid) is a major compound in the three layers that have shown a growth inhibitory effect against Gram-negative, but not Gram-positive, bacteria.